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We thank the DNA Sequencing Facility at the University of Utah, supported in part by NCI grant #5p30CA42014. This work was supported in part by a grant to P.K.B. ; from the University of Utah Research Foundation, Funding Initiative Seed Grant Program 1999 ; , in part by Grant GM48677 from NIH and by support from Cognetix, Inc., Salt Lake City, Utah.
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DRAXIMAGE is now focused on developing products that it believes will replace or improve upon several products that are currently in the Nuclear Medicine marketplace, but have or will come off patent protection in key markets in the near term. One key example of such a product is Sestamibi, a lyophilized product that is widely used for Technetium-based cardiac imaging studies, a market segment not currently served by DRAXIMAGE. A second opportunity that is currently being pursued by DRAXIMAGE is the production and distribution of a "next-generation" version of a Technetium Generator, which is the source of Technetium in virtually every radiopharmacy worldwide. Nearly 90% of generators are located in radiopharmacies and the rest are in other institutions, such as hospitals and clinics. Thirdly, DRAXIS has accelerated its efforts to obtain registrations in European markets for four of its existing products that are currently approved and sold in Canada or the United States. In February 2005, DRAXIS received approval from the Dutch regulatory authority for its Kit for the Preparation of Technetium Tc99m Albumin Aggregated Injection MAA Kit ; . Approval in the Netherlands allows DRAXIMAGE to initiate the Mutual Recognition Procedure MRP ; in pursuit of further regulatory approvals for this product in several additional European Union countries. Since initial European approvals for the majority of these diagnostic imaging products are anticipated throughout the balance of 2006, DRAXIMAGE has commenced discussions with a number of potential commercial partners to initially target large markets in Germany, France, Italy, Spain and the U.K. For the medium term, the key opportunity being pursued by DRAXIMAGE is the continued development of INFECTON, a novel diagnostic radiopharmaceutical product for imaging infection. DRAXIMAGE has also identified additional new product opportunities in the area of non-radioactive contrast media that are used in the medical imaging field and is pursuing potential product development strategies to leverage both its position in the marketplace and its preferred access to appropriate production process expertise. Contrast media products are injectable liquids produced in highlyspecialized cGMP sterile production facilities, such as those in place at DRAXIS which are currently used to produce certain diagnostic imaging products marketed by DRAXIMAGE. The North American market for contrast media has been estimated to be approximately .6 billion and it is believed to be growing driven largely by the continued growth of computer tomography CT ; and enhanced magnetic resonance imaging MRI ; procedures.
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Bilateral multifocal tumors carry a germ-line mutation in the RB1 gene and develop tumors when the second normal allele is lost or mutated in the retinoblast. However, hereditary RB can also present as unilateral tumors. The sporadic RB tumors arise when both alleles are mutated in one retinoblast in an individual with normal RB1 alleles; these events are so rare that the tumor is unilateral and unifocal. It is often uncertain whether a patient has the hereditary or the sporadic form of RB because of the high proportion of new germ line mutations. It is clear, however, that while many tumors appear indistinguishable morphologically, they are in fact molecularly distinct and such molecular distinctions can be predictive of clinical outcome. Gene expression analysis is a promising tool in cancer diagnosis to provide tumor gene expression profiles that may serve as molecular fingerprints. These molecular fingerprints can be used to distinguish the more aggressive tumors from those tumors of a more indolent nature, distinguish between the hereditary or sporadic tumors as well as identify potential genes implicated in tumorigenesis, leading to potential targets for therapeutic intervention. The broad objective to improve the health status of children with RB will be achieved by combining the powerful techniques of gene expression analysis with our ongoing mutation analysis to develop a molecular profiling system that will identify critical genes controlling tumor growth, classify RB tumor subtypes thereby allowing defined prognostic evaluation with improved genetic counseling and provide a platform for drug discovery. We propose the following specific aims: Phenotypic and Genotypic assessment of patients with RB: Patients with RB will be identified, and all clinical data will be reviewed and evaluated according to the specific parameters, such as age of onset, tumor type, method of treatment etc. Genotypic assessment will be ascertained by mutation status determined by a variety of DNA analysis techniques. To test the hypothesis that gene expression analysis can identify genes critical to tumorigenesis: Using the in-house genome-wide human 60-mer oligo expression chip containing ~18.5K uniset genes, we will compare the gene expression profiles of retinoblastoma tumor tissue to those of the normal retinal tissue to assess which genes might be implicated in tumorigenesis, based on the changes in the expression profiles. Principal Investigator N. Nina Ahmad, Ph.D. Wills Eye Hospital Research Division 840 Walnut Street Philadelphia, PA 19107 Other Participating Researchers Jerry Shields, M.D. - employed by Wills Eye Hospital Larry Donoso, M.D., Ph.D. - employed by Bower Laboratories and acidophilus.
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Infectious Agent Toxoplasma gondii Potential Effect on Fetus Fetal loss Congenital Syndrome especially: chorioretinitis hydrocephalus intracerebral calcification Rate of Perinatal Transmission st 1 trimester 10-25% increased risk for severe perinatal infection ; nd 2 trimester 30-54% 3rd trimester 60-65% Maternal Screening - Routine screening not recommended. - If maternal exposure or suspect infection, contact lab Toxoplasma IgG IgM IgA. Prevention - Wash hands: before eating after handling raw meat after contact with soil cat feces. - Pregnant woman should avoid: eating raw or rare beef, pork, or lamb contact with cats of unknown feeding history cleaning litter boxes. - If mother has suspected acute toxoplasma infection in pregnancy, consult Infectious Diseases and Maternal Fetal specialist antimicrobial therapy, amniocentesis, and need for further therapy to decrease fetal effects and acitretin.
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